Mastitis and animal husbandry – high-throughput sequencing as a support tool

High-throughput sequencing technologies.

The human genome sequence has profoundly altered our understanding of biology, human diversity, and disease. The path from the first draft sequence to our nascent era of personal genomes and genomic medicine has been made possible only because of the extraordinary advancements in DNA sequencing technologies over the past 10 years. Here, we discuss commonly used high-throughput sequencing platfo...

Microrheology as a tool for high-throughput screening

Microrheology can be used for high-throughput screening of the rheological properties of sample libraries of complex fluids. Two passive techniques are particularly suitable: video microscopy and diffusing-wave spectroscopy. The techniques complement each other very well and can be applied to samples that offer different experimental challenges. We offer a thorough analysis of the strengths and...

Animal husbandry and experimental design.

If the scientist needs to contact the animal facility after any study to inquire about husbandry details, this represents a lost opportunity, which can ultimately interfere with the study results and their interpretation. There is a clear tendency for authors to describe methodological procedures down to the smallest detail, but at the same time to provide minimal information on animals and the...

SnapShot: High-Throughput Sequencing Applications

STEAK: A specific tool for transposable elements and retrovirus detection in high-throughput sequencing data

The advancements of high-throughput genomics have unveiled much about the human genome highlighting the importance of variations between individuals and their contribution to disease. Even though numerous software have been developed to make sense of large genomics datasets, a major short falling of these has been the inability to cope with repetitive regions, specifically to validate structura...